bone marrow in old mdx mice: Dr. Zhang report

[David Feder]

BM stem cell transplantation rescues pathophysiologic features of aged dystrophic mdx muscle.
Zhang C, Zhang Y, Feng S, Yao X.
Department of Neurology, The First Affiliated Hospital of Sun Yat-Sen University. Guangzhou. China.

Background The value of transplantation of BM stem cells in aged (12-month-old) mdx was evaluated because it is thought to be a more ideal model for studying the praxiology of Duchenne muscular dystrophy (DMD). The possible mechanisms of stem cell differentiation were then discussed. Methods BM was isolated from 8-10-week-old male C57 BL/10 mice. After injecting BM cells into 12-month-old female mdx mice through the tail vein, the expression of dystrophin and MyoD was detected at different time points by immunofluorescence staining, RT-PCR and Western blot. Results The C57 male mice donor-specific and Y-chromosome-specific sequence could be detected in all female aged mdx mice, implying the success of the transplantation. Expression of dystrophin and MyoD was detected and increased over time. Discussion BM cells were recruited to the muscle and partially restored specific pathophysiologic features of the dystrophic muscle in aged mdx mice. Muscle differentiation of BM cells recapitulated embryonic myogenesis.

Cytotherapy. 2007;9(1):44-52.

www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17354101&query_hl=1&itool=pubmed_docsum

[Andre]

Hello David,

do you know Dr. Zhang and its research.
He co-operates with others?

Another question to exon skipping.
It's correct that for each AON (every Exon) clinical trial Phase I and II is necessary?
Because the chemical composition is differently for each AON.

André

[David Feder]

Dr. Zhang don't answer my e-mails. I konow some researchers that try any contact. I don't know if the had success in this contact. Probably each AON need trials (phase I and II)
David

[Glen]

David, this statement seems like a big deal to me "Muscle differentiation of BM cells recapitulated embryonic myogenesis."

Do you think th something that can happen systemically? What about in the heart?

Thanks,

Glen

[David Feder]

The summary don't give the porcentage of muscle cells that present dystrophin expression. I believe that cardiac muscle cells can be treated with the same BM stem cells. Other researchers can reproduce this results and Dr. Zhang must open the informations to other teams. This is the principle of good science.

[hb]

Hello David,
Does Adult Bone marrow Stem cell work on MD?? Has this fact established in the mice study??... b'coz other than Dr. Zhang no other doctors/scientists are exploring these field, (to my Knowledge).

hb