Bone marrow transplantation don't work in mdx mice

[David Feder]

Bone marrow transplantation improves outcome in a mouse model
of congenital muscular dystrophy
Hiroki Hagiwaraa, Yutaka Ohsawaa, Shoji Asakurab, Tatsufumi Murakamia, akanori Teshimac, Yoshihide Sunadaa - Japan
Abstract We examined whether pathogenesis in dystrophin-
deficient (mdx) mice and laminin-a2-deficient (dy) mice is ame-
liorated by bone marrow transplantation (BMT). Green fluores-
cent protein (GFP) mice were used as donors. In mdx mice,
BMT failed to produce any significant differences in muscle
pathology, although some GFP-positive fibers with restored dys-
trophin expression were observed. In contrast, in the dy mice,
BMT led to a significant increase in lifespan and an increase in growth rate, muscle strength, and respiratory function. We
conclude that BMT improved outcome in dy mice but not mdx
mice.

IN PRESS: FEBS, 2006

[David Feder]

In China bone marrow transplantation work:

www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16733899&itool=pubmed_DocSum

Dystrophin expression in mdx mice after bone marrow stem cells transplantation
OBJECTIVE: To investigate the dynamic changes of dystrophin expression in mdx mice after bone marrow stem cells transplantation. METHODS: The bone marrow stem cells of C57 BL/6 mice (aged 6 to 8 weeks) were injected intravenously into the mdx mice (aged 7 to 9 weeks), which were preconditioned with 7Gy gamma ray. The amount of dystrophin;expression in gastrocnemius was detected by immunofluorescence, reverse transcription-polymerase chain reaction and Western blot at week 5, 8, 12 and 16 after transplantation. RESULTS: At week 5 after bone marrow stem cells transplantation, the dystrophin expression detected in mdx mice were very low; however, its expression increased along with time. At week 16 week, about 12% muscle cells of all transplanted mice expressed dystrophin. There were less centrally placed myonuclei than the control mdx mice, whereas peripheral myonuclei increased. CONCLUSIONS: After having been injected into mdx mice, the allogenic bone marrow stem cells have a trend to reach the injured muscle tissues and differentiate to fibers that can express dystrophin and the expression increased with time. The bone marrow stem cells participates in the repair and regeneration of the injured tissues permanently and constantly.

[lajos]

Hi David.
I like to ask ,if My Son can take beside losartan idebenone ?
Regards

[David Feder]

Yes, it's possible to use both idebenone and losartan.
David